Ctla4 Rabbit Monoclonal Antibody [Clone ID: 9D9]

CAT#: TA386194

Recombinant Anti-CTLA-4 (Clone 9D9)

Specifications

Product Data

• Clone Name: 9D9
• Applications: IF
• Reactivities: Mouse
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: The antibody was raised against CTLA-4.
• Specificity: The antibody is specific for CTLA-4.
  
  The antibody has been used to block CTLA-4 and has been shown to enhance T-cell immunity against tumours (Quezada et al, 2006). 9D9 also has an anti-B16/BL6 melanoma effect in non-irradiated tumour-bearing recipients when given as combinatorial therapy with Gvax (Peggs et al, 2009). Selby et al. (PMID: 24777248) show how conversion of the original mouse IgG2b to a mouse IgG2a greatly increases anti-tumor activity of the 9D9 antibody in both MC38 (C57BL/6 mice) and CT26 (BALB/c mice) models. This mechanism both involves blockade of CTLA4 as well as depletion of tumor-infiltrating regulatory T-cells. Fc Silent antibodies were shown to have reduced anti-tumor activity as they did not deplete tumor infiltrating regulatory T-cells, however they lead to expansion of peripheral Tregs.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: cytotoxic T-lymphocyte-associated protein 4
• Database Link:
  Entrez Gene 12477 Mouse
  UniProt ID P09793
• Synonyms: CD; CD28; CD152; CELIAC3; CTLA-4; GRD4; GSE; ICOS; IDDM12