Ptprc Rat Monoclonal Antibody [Clone ID: RA3-6B2]

CAT#: TA386209

Recombinant Anti-CD45R (Clone RA3-6B2)

Specifications

Product Data

• Clone Name: RA3-6B2
• Applications: FC, IF, IHC
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
• Specificity: This antibody is specific for murine CD45R antigen which is expressed on B lymphocytes throughout their development from early pro-B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R mAb clone RA3-6B2 specifically recognizes the exon A–restricted isoform of mouse CD45.
  
  This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to understand the role of PD-1 in maintaining islet-specific tolerance in type 1 diabetes (Martinov et al, 2016), to investigate how Staphylococcus aureus enterotoxins spread systemically and trigger inflammatory cascades (Svedova et al, 2016), to highlight the physiologic importance of cell-type specific balancing of AhR/AhRR expression in response to microbial, nutritional and other environmental stimuli (Brandstätter et al, 2016), and to examine the role of FBXW7 in suppression of cancer metastasis in a non-cell-autonomous manner (Yumimoto et al, 2015).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: protein tyrosine phosphatase, receptor type, C
• Database Link:
  Entrez Gene 19264 Mouse
  UniProt ID P06800
• Synonyms: B220; CD45; CD45R; glycoprotein; GP180; L-CA; LCA; LY5; T200