IL5 Rabbit Monoclonal Antibody [Clone ID: 2000]

CAT#: TA386251

Recombinant Anti-IL-5 (Clone 2E3)

Product Images

Flow-cytometry using the Anti-IL-5 antibody 2E3 (Ab01127). Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 2E3 (TA386251, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: 2000
• Applications: ELISA, Neutralize
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising CAF1 mice with human recombinant IL-5.
• Specificity: This antibody is specific for human rIL-5, and does not cross-react with human rIL-1α, rIL-lβ, rIL-4, rIL-8, rM-CSF, or rTGF-α.
  
  This antibody has been show to inhibit the binding of human rIL-5 to the alpha chain of the IL-5 receptor (Ames et al, 1995). This antibody inhibits the proliferation of murine B13 cells in response to human IL-5 (Ames et al, 1995).Neutralising antibodies against IL-5 represent a potential therapeutic strategy for the treatment of eosinophilia. The specificity of this antibody for human IL-5 has been confirmed in ELISA analysis (Ames et al, 1995).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: interleukin 5
• Database Link:
  Entrez Gene 3567 Human
  UniProt ID C9JQP9
• Synonyms: EDF; IL-5; interleukin-5; TRF