Il9 Rabbit Monoclonal Antibody [Clone ID: D9302C12]

CAT#: TA386283

Recombinant Anti-IL-9 (Clone D9302C12)

Product Images

Flow-cytometry using the Anti-IL-9 antibody D9302C12 (Ab01260). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (TA386283, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: D9302C12
• Applications: Bl, FC
• Reactivities: Mouse
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG2 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising Armenian hamsters with Baculovirus-expressed, recombinant murine IL-9.
• Specificity: This antibody is specific for murine IL-9.
  
  In vitro, this antibody has inhibitory effects on IL-9 bioactivity, and inhibits the differentiation of Th17 cells and consequent production of IL-17 (Li et al, 2010). This antibody is also able to neutralise IL-9 in vivo (Li et al, 2010). Administration of this antibody suppresses acute experimental autoimmune encephalomyelitis (EAE), with an observed reduction in MOG-specific Th1 and Th17 cells in the periphery and the CNS (Li et al, 2010).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: interleukin 9
• Database Link:
  Entrez Gene 16198 Mouse
  UniProt ID P15247
• Synonyms: HP40; IL-9; P40