Il17f Rabbit Monoclonal Antibody [Clone ID: 8F5.1A9]

CAT#: TA386291

Recombinant Anti-IL-17F (Clone 8F5.1A9)

Product Images

Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9 (Ab01264). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 8F5.1A9 (TA386291, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: 8F5.1A9
• Applications: FC
• Reactivities: Mouse
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising mice with murine IL-17F coupled to ovalbumin to generate a highly immunogenic complex.
• Specificity: This antibody recognises murine IL-17F.
  
  This antibody has been used to analyse IL-17F expression by tongue cells through intracellular staining, evaluated using flow cytometry (Sparber et al, 2018).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: interleukin 17F
• Database Link:
  Entrez Gene 257630 Mouse
  UniProt ID Q7TNI7
• Synonyms: IL-17F; IL-24; IL24; Interleukin-24; ML-1; ML1