RAP Rabbit Monoclonal Antibody [Clone ID: 7F1]

CAT#: TA386313

Recombinant Anti-RAP (Clone 7F1)

Product Images

Flow cytometry using the anti-RAP antibody 7F1 (Ab01382). HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG1 version of 7F1 (TA386313, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-RAP antibody (Ab01382) Staining in HeLa(A) (0.1ug/ml), A549 (B) (0.01ug/ml), HepG2 (C) (0.01ug/ml), HEK293 (D) (0.03ug/ml), MCF7 (E) (0.03ug/ml), Caco-2 (F) (0.001ug/ml) and U251 (G) (0.001ug/ml) cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAP (TA386313) at 0.5 µg/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: 7F1
• Applications: ELISA, IHC, IP, WB
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising mice with human placental RAP.
• Specificity: This antibody is specific for human RAP. It does not cross-react with rabbit or murine RAP.
  
  This antibody has been used to assess human RAP expression by RAP-transfected H4 cells, derived from human neuroglioma cells, in immunostaining analysis (Kinoshita et al, 2001). It has also been used in immunohistochemical analysis of paraffin-embedded human kidney tissue sections (Kounnas et al, 1992), and in ELISA analysis to detect RAP binding (Medh et al, 1995; Kounnas et al, 1993). Additionally, this antibody has been used to immunoprecipitate RAP, and in western blot analysis of purified B-amyloid protein and RAP mixtures (Kerr et al, 2010).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.