CD45 (PTPRC) Rabbit Monoclonal Antibody [Clone ID: OTH-74D4]

CAT#: TA386348

Recombinant Anti-CD45RA (Clone OTH-74D4)

Product Images

Flow cytometry using the Anti-CD45RA antibody OTH-74D4 (Ab01502). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OTH-74D4 (TA386348, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: OTH-74D4
• Applications: FC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising mice with an EBV-transformed B cell line from a patient with MHC class II deficiency.
• Specificity: OTH-74D4 antibody recognises CD45RA, which is an CD45 isoform containing only protein A region.The CD45RA antigen is expressed mostly on naïve CD4+, CD8+ and gd+ T and on most B and NK lymphocytes. Generally the CD45RA antigen density decreases upon activation.
  
  OTH-74D4 antibody is recommended for the flow cytometric identification of CD45RA. The CD45RA expression on immune cells is used as one of the key surface antigen to determine the functional status and also to define the end-stage of human T cells.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: protein tyrosine phosphatase, receptor type C
• Database Link:
  Entrez Gene 5788 Human
  UniProt ID P08575
• Synonyms: B220; CD45; CD45R; glycoprotein; GP180; L-CA; LCA; LY5; T200