CXCR1 Rabbit Monoclonal Antibody [Clone ID: SE2]

CAT#: TA386404

Recombinant Anti-IL-8RA (Clone SE2)

Product Images

Flow cytometry using the Anti-IL-8RA antibody SE2 (Ab01582). Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SE2 (TA386404, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-IL-8RA antibody SE2 (Ab01582) Human lymph node tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SE2 (TA386404) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: SE2
• Applications: FC, IHC, WB
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of IL-8RA (amino acids 1-30).
• Specificity: SE2 antibody is specific for IL-8RA and does not corss-react with IL-8RB. IL-8RA is a receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils.
  
  This antibody is recommended for the detection and analysis of IL-8RA expression. One group used it to identify IL-8RA on the surface of human granulocytes via FACS (Zahn et al., 1997). Importantly, SE2 antibody was specific to IL-8RA-expressing cells only. Subsequent flow cytometric analysis with this antibody led to the characterization of IL-8RA density on human leukocytes (Zahn et al., 1997).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: C-X-C motif chemokine receptor 1
• Database Link:
  Entrez Gene 3577 Human
  UniProt ID P25024
• Synonyms: Il8ra