CXCR2 Rabbit Monoclonal Antibody [Clone ID: HC2]

CAT#: TA386407

Recombinant Anti-IL-8RB (Clone HC2)

Product Images

Flow cytometry using the Anti-IL-8RB antibody HC2 (Ab01583). Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of HC2 (TA386407, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: HC2
• Applications: FC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunizing mice with human IL-8 (interleukin-8) receptor, type B.
• Specificity: HC2 recognizes human C-X-C chemokine receptor type 2, (CXCR2). CD182 is a 360 amino acid multipass membrane glcycoprotein and is expressed by neutrophils and monocytes, as well as by some subsets of T cells and endothelial cells.
  
  This antibody can be used in the detection and analysis of IL-8RB via flow cytometry (Zahn et al., 1997).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: C-X-C motif chemokine receptor 2
• Database Link:
  Entrez Gene 3579 Human
  UniProt ID P25025
• Synonyms: CD128; CDw128; Cmkar2; Gpcr16; IL-8rb; IL-8Rh; IL8RA; Il8rb; mIL-8RH