CD137 (TNFRSF9) Rabbit Monoclonal Antibody [Clone ID: 4B4-1-1]

CAT#: TA386441

Recombinant Anti-4-1BB (Clone 4B4-1-1)

Product Images

Western Blot using anti-4-1BB antibody 4B4-1-1 (Ab01689) Human thymus(A), lymph node(B) and tonsil(C) tissue lysate (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4B4-1-1 (TA386441) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: 4B4-1-1
• Applications: ELISA, FC, IHC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: The original antibody was generated by immunizing BALB/c mice with a fusion protein of human 4-1BB antigen and glutathione s transferase (GST).
• Specificity: This antibody binds to the human 4-1BB, which is expressed on the surface of activated T cells.
  
  The antibody has good binding affinity for Con A-activated T cells and has an immunosuppressive activity towards lymphocyte proliferation in the in vitro mixed lymphocyte reaction. The binding of antibody to surface expressed 4-1BB molecule was assessed using flow cytometry and ELISA was used to check whether the cell culture had binding affinity to 4-1BB (Kim et al., 1995). The interaction of this antibody with CD137 is reported to induce an increase in TNF-α production by monocytes. This antibody competes with mAb M121 for binding to CD137 (PMID: 10607752). This antiboy also induces the production of IFN-γ in NK cells in presence of IL-15 (PMID: 20008791).This antibody can be used in blocking assays and staining methods (PMID: 14746806). Humanized versions of this antibody (Hz4B4-1) and (Hz4B4-2) showed a 1.5 fold and 7.3 fold increase and in binding affinity for 4-1BB as compared to their parental mouse antibody. Additionally, H4B4 mediated antibody-dependent cellular toxicity of activated human peripheral blood T cells and CEM cells in a dose dependent manner (PMID: 11186149). Humanized version of this antibody is available on request.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: tumor necrosis factor receptor superfamily member 9
• Database Link:
  Entrez Gene 3604 Human
  UniProt ID Q07011
• Synonyms: 4-1BB; CD137; CDw137; ILA; MGC2172