HP1 alpha (CBX5) Rabbit Monoclonal Antibody [Clone ID: RAB-C133]

CAT#: TA386453

Recombinant Anti-CBX5 (Clone RAB-C133)

Product Images

Flow cytometry using the Anti-CBX5 antibody RAB-C133 (Ab01703). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of RAB-C133 (TA386453, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-CBX5 antibody RAB-C133 (Ab01703). Nuclear lysates of HeLa(A), HEK293(B), A431(C), MCF7(D), Jurkat(E) and K562(F) cells (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C133 (TA386453) at 0.003 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: RAB-C133
• Applications: ChIP, ELISA
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing CBX5 protein under non-denaturing conditions.
• Specificity: This antibody recognizes CBX5 (Chromobox protein homolog 5). It binds to a folded domain, amino acids 18-75. CBX5 is a component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression.
  
  This antibody has been used and recommended for the following methods: ELISA; IP-MS; ChIP.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: chromobox 5
• Database Link:
  Entrez Gene 23468 Human
  UniProt ID P45973
• Synonyms: HP1; HP1-ALPHA; HP1A; HP1Hs-alpha