L3MBTL1 Rabbit Monoclonal Antibody [Clone ID: RAB-C213]

CAT#: TA386463

Recombinant Anti-L3MBTL1 (Clone RAB-C213)

Product Images

Flow cytometry using the Anti-L3MBTL1 antibody RAB-C213 (Ab01709). Paraformaldehyde fixed Kelly cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of RAB-C213 (TA386463, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: RAB-C213
• Applications: ChIP, ELISA, FC, IF
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing L3MBTL1 protein under non-denaturing conditions.
• Specificity: This antibody recognizes L3MBTL1 (Lethal(3)malignant brain tumor-like protein 1). It binds to a folded domain, amino acids 200-530. L3MBTL1 is a Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications.
  
  This antibody has been used and recommended for the following methods: ELISA; IF; spiked IP; IP-MS; ChIP; FC.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: l(3)mbt-like 1 (Drosophila)
• Database Link:
  Entrez Gene 26013 Human
  UniProt ID Q9Y468
• Synonyms: C630004G01; L3mbtl; mKIAA0681