FOXP1 Rabbit Monoclonal Antibody [Clone ID: RAB-S37]

CAT#: TA386481

Recombinant Anti-FOXP1 (Clone RAB-S37)

Product Images

Flow cytometry using the Anti-FOXP1 antibody RAB-S37 (Ab01718). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of RAB-S37 (TA386481, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-FOXP1 antibody RAB-S37 (Ab01718). Human skeletal muscle tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-S37 (TA386481) at 0.003 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: RAB-S37
• Applications: ELISA, FC, IF
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing FOXP1 protein under non-denaturing conditions.
• Specificity: This antibody recognizes FOXP1 (Forkhead box protein P1). It binds to a folded domain, amino acids 302-541. FOXP1 is a transcriptional repressor that plays an important role in the specification and differentiation of lung epithelium.
  
  This antibody has been used and recommended for the following methods: ELISA; spiked-IP; IF; FC.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: forkhead box P1
• Database Link:
  Entrez Gene 27086 Human
  UniProt ID Q9H334
• Synonyms: 12CC4; FLJ23741; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF1