CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: IV.3]

CAT#: TA386513

Recombinant Anti-CD32 (Clone IV.3)

Specifications

Product Data

• Clone Name: IV.3
• Applications: ELISA, IF, IP
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: The original antibody was generated against the FcR of K562 cells.
• Specificity: This antibody recognizes and binds CD32a.
  
  This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic “FCGR2A” mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: Fc fragment of IgG receptor IIa
• Database Link:
  Entrez Gene 2212 Human
  UniProt ID P12318
• Synonyms: CD32; CD32A; CDw32; Fc-gamma-RIIa; FCG2; FcGR; FCGR2; FCGR2A1; FcRII-a; IGFR2; MGC23887; MGC30032