CD163 Mouse Monoclonal Antibody [Clone ID: 2A10 (2A10/11)]

CAT#: TA386520

Recombinant Anti-CD163 (Clone 2A10 (2A10/11))

Specifications

Product Data

• Clone Name: 2A10 (2A10/11)
• Applications: ELISA, FC, IF
• Host: Mouse
• Isotype: IgG1, lambda
• Clonality: Monoclonal
• Immunogen: The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.
• Specificity: This antibody recognizes and binds porcine CD163.
  
  This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID: 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID:23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID:21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID: 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID: 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Pérez et al, 2008).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Database Link:
  UniProt ID Q2VL90
• Synonyms: M130; MM130