IL3RA Mouse Monoclonal Antibody [Clone ID: 7G3]

CAT#: TA386524

Recombinant Anti-CD123 (Clone 7G3)


USD 523.00

5 Weeks*

Size
    • 200 ug

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Specifications

Product Data
Clone Name 7G3
Applications FC, IP, WB
Reactivities Human
Host Mouse
Isotype IgG2a, kappa
Clonality Monoclonal
Immunogen The original antibody was generated by intraperitoneally immunizing BALB/c mice with 5 X lo6 COS cells transfected with the IL-3R a-chain in combination with adjuvant peptides.
Specificity This antibody specifically binds to the IL-3R alpha-chain and completely abolishes its function. IL-3R is expressed on myeloid, lymphoid, and vascular endothelial cells, where it transduces IL-3-dependent signals leading to cell activation.

This antagonist antibody binds IL-3Rα and blocks IL-3 binding and IL-3-mediated functions. mAb 7G3 immunoprecipitated and recognized in western blots the IL-3R α chain expressed by transfected cells and bound to primary cells expressing IL-3Rα. The expression of IL-3Rα chain on various cells types was analysed by flow cytometry using this antibody (PMID: 8547680). It is reported that 7G3 inhibits IL-3Rα and βc dimerization (PMID: 8649415). This antibody was used in the study of neutrophils expressing IL-3 receptors which are activated by granulocyte-macrophage colony-stimulating factor or GM-CSF (PMID: 7579364). The original 7G3, mouse IgG2a antibody inhibited IL-3-mediated intracellular signaling of isolated acute myeloid leukemia (AML) CD34+ CD38− cells in vitro and reduced their survival (PMID: 19570512). Humanized version of this antibody CSL362 is available on request. CSL362, retains the ability to neutralise IL-3 and has enhanced affinity for the FcγRIIIa (CD16) on NK cells. In vitro studies have demonstrated that the increased affinity for CD16 correlates with greater antibody-dependent cell-mediated cytotoxicity (ADCC) against CD123 expressing cell lines (Busfield et al., 2012). CSL362 is being accessed for use in the treatment of myeloid leukemia (PMID: 24363400, 2524888, 26130514).
Formulation PBS with 0.02% Proclin 300.
Concentration lot specific
Conjugation Unconjugated
Storage Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Stability 3 years from dispatch.
Gene Name interleukin 3 receptor subunit alpha
Synonyms CD123; hIL-3Ra; IL-3R-alpha; IL3R; IL3RAY; IL3RX; IL3RY; MGC34174
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.