IL1 beta (IL1B) Mouse Monoclonal Antibody

CAT#: TA388899

IL-1 beta Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 0.25-0.50 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human IL-1 beta when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Human IL-1beta as the detection antibody at a concentration of approximately 0.25-0.50 μg/ml.
  Western Blot
  To detect Human IL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-1beta is 2.0-4.0 ng/lane, under reducing conditions and 4.0-8.0 ng/lane, under non-reducing conditions.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Produced in BALB/c mice immunized with highly pure Recombinant Human IL-1beta. Anti-Human IL-1 beta-specific antibody was purified from cell culture by Protein G affinity chromatography.
• Concentration: lot specific
• Purification: IL-1 beta-specific antibody was purified from cell culture by Protein G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 1 beta
• Database Link:
  Entrez Gene 3553 Human
  UniProt ID P01584
• Background: IL-1 beta is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. Recombinant Human IL-1 beta is a 17.3 kDa protein containing 153 amino acid residues.
• Synonyms: catabolin; IL-1; IL-1B; IL1-BETA; IL1F2; pro-interleukin-1-beta