IL3 Mouse Monoclonal Antibody

CAT#: TA388901

IL-3 Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 8pg/ml of Recombinant Human IL-3 when used with ProSci’s Biotinylated Antigen Affinity Purified Anti-Human IL-3 as the detection antibody at a concentration of approximately 0.50- 1.0 μg/ml.
  Western Blot
  To detect Human IL-3 by Western Blot analysis, this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-3 is 2.0-4.0 ng/lane, under reducing and non-reducing conditions.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Produced in mice immunized with highly pure Recombinant Human IL-3. Anti-Human IL-3-specific antibody was purified from tissue culture supernatant by Protein G affinity chromatography.
• Concentration: lot specific
• Purification: IL-3-specific antibody was purified from tissue culture supernatant by Protein G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 3
• Database Link:
  Entrez Gene 3562 Human
  UniProt ID P08700
• Background: IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.
• Synonyms: IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF