IL10 Mouse Monoclonal Antibody

CAT#: TA388905

IL-10 Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 8.0-10.0 μg/ml of this antibody will detect at least 2000pg/ml of Recombinant Human IL-10 when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human IL-10 as the detection antibody at a concentration of approximately 0.05- 0.10 μg/ml.
  Western Blot
  To detect Human IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-10 is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Produced in BALB/c mice immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography.
• Concentration: lot specific
• Purification: IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 10
• Database Link:
  Entrez Gene 3586 Human
  UniProt ID P22301
• Background: IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells. Recombinant Human IL-10 is an 18.6 kDa protein of 161 amino acid residues.
• Synonyms: CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF