Csf2 Rabbit Polyclonal Antibody

CAT#: TA388924

GM-CSF Antibody (biotin)

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine GM-CSF as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mGM-CSF.
  Western Blot
  To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Murine GM-CSF. Anti-Murine GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: lot specific
• Purification: GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: colony stimulating factor 2 (granulocyte-macrophage)
• Database Link:
  Entrez Gene 12981 Mouse
  UniProt ID P01587
• Background: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Murine GM-CSF is a 14.2 kDa globular protein consisting of 125 amino acid residues.
• Synonyms: CSF; GM-CSF; GMCSF; MGC131935; MGC138897; molgramostin; sargramostim