Neurotrophin 3 (NTF3) Rabbit Polyclonal Antibody

CAT#: TA388927

NT-3 Antibody (biotin)

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect hNT-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human NT-3 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNT-3.
  Western Blot
  To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNT-3. Anti-Human NT-3 specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: lot specific
• Purification: NT-3 specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: neurotrophin 3
• Database Link:
  Entrez Gene 4908 Human
  UniProt ID P20783
• Background: NT-3 is a neurotrophic factor structurally related tobeta-NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous system, and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant Human NT-3 is a noncovalently linked homodimer of two 13.6 kDa polypeptide monomers (240 total amino acid residues).
• Synonyms: HDNF; MGC129711; NGF-2; NGF2; NT-3; NT3