Il13 Rabbit Polyclonal Antibody

CAT#: TA388938

IL-13 Antibody (biotin)

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Rat IL-13 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti- Rat IL-13 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-13.
  Western Blot
  To detect Rat IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-13 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat IL-13. Anti-Rat IL-13-specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: lot specific
• Purification: IL-13 specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 13
• Database Link:
  Entrez Gene 116553 Rat
  UniProt ID P42203
• Background: IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1b, TNF-a, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 are cross-species reactive. Recombinant Rat IL-13 a 12.3 kDa protein consisting of 113 amino acid residues.
• Synonyms: ALRH; BHR1; IL-13; MGC116786; MGC116788; MGC116789; NC30; P600