Cxcl12 Rabbit Polyclonal Antibody

CAT#: TA388949

SDF-1 alpha (CXCL12) Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Rat SDF-1 alpha by sandwich ELISA (using 100ul/well antibody solution), a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat SDF-1 alpha as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SDF-1 alpha.
  Western Blot
  To detect Rat SDF-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat SDF-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat SDF-1 alpha. Anti-Rat SDF-1 alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1 alpha matrix.
• Concentration: lot specific
• Purification: SDF-1 alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1 alpha matrix
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: C-X-C motif chemokine ligand 12
• Database Link:
  Entrez Gene 24772 Rat
• Background: SDF-1 alpha andbeta are stromal-derived, CXC chemokines that signal through the CXCR4 receptor. SDF-1alpha andbeta chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV-suppressive activity in cells expressing the CXCR4 receptor. Human and murine SDF-1 proteins act across species. SDF-1 alpha andbeta contain the four highly conserved cysteine residues present in CXC chemokines. The mature SDF-1 alpha protein is the result of alternative splicing of the SDF-1 gene and contains 68 amino acid residues. Recombinant Rat SDF-1alpha is a 7.9 kDa protein containing 68 amino acid residues.
• Synonyms: hIRH; hSDF-1; PBSF; SCYB12; SDF-1; SDF-1a; SDF-1b; SDF1; SDF1A; SDF1B; TLSF-a; TLSF-b; TPAR1