EPO Rabbit Polyclonal Antibody

CAT#: TA388955

EPO Antibody


USD 350.00

5 Days*

Size
    • 5 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human EPO as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of Recombinant Human EPO.
Western Blot
To detect Human EPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography employing an immobilized Human EPO matrix.
Concentration lot specific
Purification EPO-specific antibody was purified by affinity chromatography employing an immobilized Human EPO matrix
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name erythropoietin
Background Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. ProSci’s Human EPO contains 166 amino acid residues and has a calculated molecular weight of approximately 18.4 kDa. As a result of glycosylation, Recombinant Human EPO migrates with an apparent molecular mass of 37.0 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
Synonyms EP; epoetin; erythropoietin; MGC138142; MVCD2
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.