EWSR1 Mouse Monoclonal Antibody [Clone ID: 5H7]

CAT#: TA389007

Anti-EWS Antibody

Specifications

Product Data

• Clone Name: 5H7
• Applications: ICC, IHC, WB
• Recommended Dilution: WB: 1:1000
  ICC: 1:1000
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length recombinant human EWS expressed in and purified from E. coli.
• Specificity: Specific for endogenous levels of the ~85 kDa EWS protein.
• Formulation: PBS + 50% glycerol and 5 mm NaN3
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 85
• Gene Name: EWS RNA binding protein 1
• Database Link:
  Entrez Gene 2130 Human
  UniProt ID Q01844
• Background: The Ewing Sarcoma Breakpoint Region 1 (EWSR1) gene encodes the EWS protein, which along with FUS and TAF15 comprises the FET family of RNA-binding proteins. EWS is primarily expressed in the nucleus, but has also been found in the cytoplasm of secretory cell types (Andersson et al., 2008). Defects of EWSR1 are known to cause Ewing sarcoma and have been implicated in amyotrophic lateral sclerosis (ALS) (Svetoni et al., 2016). Recently, EWS has been shown to directly interact with oncogenic ETS transcription factors in the promotion of prostate cancer (Kedage et al., 2016).
• Synonyms: bK984G1.4; EWS
• Note: Protein G purified from culture supernatant.