MECP2 Mouse Monoclonal Antibody [Clone ID: 5H12]

CAT#: TA389018

Anti-MeCP2 Antibody

Specifications

Product Data

• Clone Name: 5H12
• Applications: ICC, IHC, WB
• Recommended Dilution: WB: 1:2000
  WB Brain: 1:2000
  ICC: 1:1000-1:5000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant MeCP2
• Specificity: Specific for endogenous levels of the MeCP2 protein. Due to usually high charge, the protein runs at ~75 kDa in SDS-PAGE of mouse brain lysates.
• Formulation: PBS + 50% glycerol and 5 mM NaN3
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 75
• Gene Name: methyl-CpG binding protein 2
• Database Link:
  Entrez Gene 4204 Human
  UniProt ID P51608
• Background: MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
• Synonyms: AUTSX3; DKFZp686A24160; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT
• Note: Protein G purified culture supernatant