Phospho-DOK1 (pTyr362) Rabbit Antibody [Clone ID: WB, E]
Product Images
Other products for "DOK1"
Specifications
Product Data | |
Clone Name | WB, E |
Applications | WB |
Recommended Dilution | WB: 1:1000 |
Reactivities | Human, Mouse, Rat |
Host | Rabbit |
Isotype | IgG |
Immunogen | Phospho-Dok1 (Tyr-362) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding tyrosine 362 in human Dok1. This sequence is conserved in Dok1 from rat and mouse (Tyr-361), and has high homology to Dok2 (Tyr-337). The site is not conserved in other Dok family members. |
Specificity | This antibody was cross-adsorbed to a phosphotyrosine peptide before affinity purification using phospho-Dok1 (Tyr-362) peptide. The purified antibody detects a band at 62 kDa* corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after alkaline phosphatase treatment. Similar to Dok1 (Ser-450) antibody, this antibody also detects an 80 kDa band that has not been identified. |
Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Concentration | lot specific |
Purification | Antigen Affinity Purified |
Conjugation | Unconjugated |
Storage | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Stability | After date of receipt, stable for at least 1 year at -20°C. |
Predicted Protein Size | 62 |
Database Link | |
Background | Doks are a family of adaptor proteins that include six Dok proteins (Dok1 to Dok6), which have an N-terminal pleckstrin homology domain, a central phosphotyrosine binding domain, and a C-terminal region containing multiple tyrosine residues. When phosphorylated, these tyrosines can serve as docking sites for SH2 domain-containing proteins. Dok1 (p62dok) has been shown to bind Ras-GAP, Nck, and Csk. Several tyrosine phosphorylation sites have been identified for Dok1. One site, Tyr-362 (Tyr-361 mouse), is phosphorylated by c-Abl, is required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin. Alternatively, Dok1 activity is also regulated by serine phosphorylation. IκB Kinase β phosphorylates several serine sites including Ser-450 in vitro, and TNFα, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo. Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility. |
Note | Antigen affinity purified rabbit serum. |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
{0} Product Review(s)
0 Product Review(s)
Submit review
Be the first one to submit a review
Product Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.