Phospho-SRC (pTyr530) Mouse Antibody [Clone ID: M261]

CAT#: TA389214

Anti-c-Src (Tyr-530) [conserved site], Phosphospecific Antibody


USD 385.00

5 Days*

Size
    • 100 ul

Product Images

Specifications

Product Data
Clone Name M261
Applications WB
Recommended Dilution WB: 1:500
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Immunogen Clone (M261) was generated from a phosphopeptide containing amino acid surrounding Tyr-530 at the C-terminus of human c-Src. This sequence has high homology to the conserved site in rat and mouse c-Src, and is conserved in the c-Src family of kinases.
Specificity This antibody detects a 60 kDa* protein corresponding to c-Src on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and treated with pervanadate. This antibody also detects the conserved site in Lck (Tyr-505) in human Jurkat cells treated with pervanadate. This antibody may detect several c-Src family members phosphorylated at the conserved site for Tyr-530.
Formulation PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration lot specific
Purification Protein A Purified
Conjugation Unconjugated
Storage Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Stability After date of receipt, stable for at least 1 year at -20°C.
Predicted Protein Size 60
Background c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.
Note Protein G purified tissue culture supernatant.
Reference Data

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