EIF4EBP1 Rabbit Polyclonal Antibody

CAT#: TA392494

4E-BP1 (Phospho-Thr70) polyclonal antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000~1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Threonine 70.
• Specificity: 4E-BP1(Phospho-Thr70) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr70.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 18 kDa
• Gene Name: eukaryotic translation initiation factor 4E binding protein 1
• Database Link:
  Entrez Gene 1978 Human
  UniProt ID Q13541
• Background: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. Multiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.
• Synonyms: 4E-BP1; eIF4E-binding protein 1; EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1
• Note: For research use only, not for use in diagnostic procedure.