14-3-3 zeta (YWHAZ) Rabbit Polyclonal Antibody

CAT#: TA392879

14-3-3 ζ (phospho-S58) polyclonal antibody

Product Images

Western blot (WB) analysis of 14-3-3 ζ (phospho-S58) polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug) Lane4:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)

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  Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (phospho-S58) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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  Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (phospho-S58) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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  Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (phospho-S58) pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Histone 14-3-3 ζ around the phosphorylation site of Serine 58.
• Specificity: p-14-3-3 ζ (S58) polyclonal antibody detects endogenous levels of 14-3-3 protein zeta/delta only when phosphorylated at Ser58.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 28 kDa
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• Database Link:
  Entrez Gene 7534 Human
  UniProt ID P63104
• Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
• Synonyms: 14-3-3 protein delta; 14-3-3 protein zeta; 14-3-3 protein zeta/delta; 14-3-3 δ; 14-3-3 ζ; 1433Z; KCIP-1; KCIP 1; KCIP1; Protein kinase C inhibitor protein 1; YWHAZ
• Note: For research use only, not for use in diagnostic procedure.