Cyclin E1 (CCNE1) Rabbit Polyclonal Antibody

CAT#: TA392886

Cyclin E1 (phospho-T395) polyclonal antibody

Product Images

Western blot (WB) analysis of p-Cyclin E1 (T395) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:BV2 whole cell lysate(40ug)

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  Immunohistochemistry (IHC) analyzes of p-Cyclin E1 (T395) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Cyclin E1 around the phosphorylation site of Threonine 395.
• Specificity: p-Cyclin E1 (T395) polyclonal antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at Thr395
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 48 kDa
• Gene Name: cyclin E1
• Database Link:
  Entrez Gene 898 Human
  UniProt ID P24864
• Background: Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner.
• Synonyms: CCNE; CCNE1; G1/S-specific cyclin-E1
• Note: For research use only, not for use in diagnostic procedure.