EEF2K Rabbit Polyclonal Antibody

CAT#: TA392889

eEF2K (phospho-S366) polyclonal antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human eEF2K around the phosphorylation site of Serine 366.
• Specificity: p-eEF2K (S366) polyclonal antibody detects endogenous levels of eEF2K protein when phosphorylated at Ser366.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 105 kDa
• Gene Name: eukaryotic elongation factor 2 kinase
• Database Link:
  Entrez Gene 29904 Human
  UniProt ID O00418
• Background: eEF2K is 105 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38γ, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.
• Synonyms: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; EEF2K; Elongation factor 2 kinase
• Note: For research use only, not for use in diagnostic procedure.