RUNX1 Rabbit Polyclonal Antibody

CAT#: TA392898

RUNX1 (phospho-S249) polyclonal antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human RUNX1 around the phosphorylation site of Serine 249.
• Specificity: RUNX1 (phospho-S249) polyclonal antibody detects endogenous levels of RUNX1 protein only when phosphorylated at Ser249.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 55 kDa
• Gene Name: runt related transcription factor 1
• Database Link:
  Entrez Gene 861 Human
  UniProt ID Q01196
• Background: AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors. It is required for normal development of all hematopoietic lineages. AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2. Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner.
• Synonyms: Acute myeloid leukemia 1 protein; AML1; CBF-alpha-2; CBFA2; Core-binding factor subunit alpha-2; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; Runt-related transcription factor 1; RUNX1; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit
• Note: For research use only, not for use in diagnostic procedure.