Calnexin (CANX) Rabbit Polyclonal Antibody

CAT#: TA392912

Calnexin (phospho-S583) polyclonal antibody

Product Images

Western blot (WB) analysis of Calnexin (phospho-S583) pAb at 1:10000 to 1:50000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug)

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  Immunohistochemistry (IHC) analyzes of p-Calnexin (S583) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

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  Immunoprecipitation of L02 whole cell lysate using Calnexin (phospho-S583) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5 ) .Lane 1 is 20% input. The western blot was probed using Calnexin (phospho-S583) pAb #TA392912.“↑”（supernatant）; “↓”（deposition）

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:10000~1:50000 IHC: 1:50~1:1000 IP: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Calnexin around the phosphorylation site of Serine 583.
• Specificity: p-Calnexin (S583) polyclonal antibody detects endogenous levels of Calnexin protein only when phosphorylated at Ser583
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 90 kDa
• Gene Name: calnexin
• Database Link:
  Entrez Gene 821 Human
  UniProt ID P27824
• Background: Calnexin and Calregulin (also called Calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum—Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
• Synonyms: Calnexin; CANX; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
• Note: For research use only, not for use in diagnostic procedure.