EGFR Rabbit Polyclonal Antibody

CAT#: TA392923

EGFR (phospho-Y992) polyclonal antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 992.
• Specificity: p-EGFR (Y992) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr992 (removal of the signal peptide).
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 175 kDa
• Gene Name: epidermal growth factor receptor
• Database Link:
  Entrez Gene 1956 Human
  UniProt ID P00533
• Background: Epidermal growth factor mediates its effects on cell growth through its interaction with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGFα to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy-terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptorgulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
• Synonyms: EC=2.7.10.1; EGFR; Epidermal growth factor receptor; ERBB; ERBB1; HER1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
• Note: For research use only, not for use in diagnostic procedure.