PKC alpha (PRKCA) Rabbit Polyclonal Antibody

CAT#: TA392935

PKC α (phospho-T505) polyclonal antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human PKC α around the phosphorylation site of Threonine 505.
• Specificity: p-PKC α (T505) polyclonal antibody detects endogenous levels of PKC α protein only when phosphorylated at Thr505.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 80 kDa
• Gene Name: protein kinase C alpha
• Database Link:
  Entrez Gene 5578 Human
  UniProt ID P17252
• Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.
• Synonyms: PKC-A; PKC-alpha; PKCA; PRKACA; PRKCA; Protein kinase C alpha type
• Note: For research use only, not for use in diagnostic procedure.