H3C14 Rabbit Polyclonal Antibody

CAT#: TA397478

Histone H3 K9me3/phospho T6 Antibody

Specifications

Product Data

• Applications: ChIP, IF, IHC, WB
• Recommended Dilution: WB: 1:500
  IHC: 1:200
  IF: 1:200
  CHiP: 2-5µg/million cells
• Reactivities: C. elegans, Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Histone H3 [Trimethyl Lys9, p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated/phosphorylated peptide surrounding Lysine 9/Threonine 6 of human Histone H3.2.
• Specificity: Anti-Histone H3 [Trimethyl Lys9, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Expiration date is one (1) year from date of receipt.
• Gene Name: histone cluster 2, H3c
• Database Link:
  Entrez Gene 333932 HumanEntrez Gene 126961 Human
  UniProt ID Q71DI3
• Background: H3K9 methylations are a conserved hallmark of heterochromatin binding domains. The trimethyl K9 version of histone H3, when coupled with a T6 phosphorylation, is related to the Oct4 and Nanog proteins in embryoid bodies. This association seems to indicate a role for this modified histone in cellular differentiation in development. The formation of this modification seems to be a result of a complicated multiple silencing process for downstream genes, which is RNAi independent. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
• Synonyms: rabbit anti-Histone H3 trimethyl Lys9 pT6 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9me3/pT6
• Note: Anti-Histone H3 [Trimethyl Lys9, p Thr6] antibody is tested for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Reference Data

• Protein Pathways: Systemic lupus erythematosus