STAT4 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2A2]

CAT#: TA500005AM

Anti-Stat4 mouse monoclonal antibody, clone OTI2A2 (formerly 2A2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Stat4 (RC206892, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Stat4. Positive lysates LY401095 (100ug) and LC401095 (20ug) can be purchased separately from OriGene.

• 

  STAT4 antibody ( 2A2 ) at 1:100 dilution + Hela cell lysate

• 

  Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-Stat4 (TA500005) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Stat4 (TA500005) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Specifications

Product Data

• Clone Name: OTI2A2
• Applications: IHC, WB
• Recommended Dilution: WB: 1:100, IHC 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 633-652 of human Stat4
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.8 kDa
• Gene Name: signal transducer and activator of transcription 4
• Database Link:
  NP_003142
  Entrez Gene 20849 MouseEntrez Gene 367264 RatEntrez Gene 6775 Human
  UniProt ID Q14765
• Background: Stat4 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
• Synonyms: SLEB11

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway