Hex (HHEX) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3C4]

CAT#: TA500026BM

Anti-Hex (Hex) mouse monoclonal antibody, clone OTI3C4 (formerly 3C4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HHex (RC204815, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HHex. Positive lysates LY419139 (100ug) and LC419139 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HHex monoclonal antibody at 1:50 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Hex antibody (3C4) at 1:100 dilution + Lysates from HEK-293T cells transfected with human Hex expression vector (Cat# RC204815)

• 

  Anti-HHex mouse monoclonal antibody (TA500026) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY HHex (RC204815)

• 

  Immunofluorescent staining of HeLa cells using anti-HHex mouse monoclonal antibody (TA500026).

Show all 5 images

Specifications

Product Data

• Clone Name: OTI3C4
• Applications: IF, WB
• Recommended Dilution: WB: 1:50 - 1:100, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 100-270 of human Hex
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa (experimental 44 kDa)
• Gene Name: hematopoietically expressed homeobox
• Database Link:
  NP_002720
  Entrez Gene 15242 MouseEntrez Gene 79237 RatEntrez Gene 3087 Human
  UniProt ID Q03014
• Background: Hex is a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation.
• Synonyms: HEX; HMPH; HOX11L-PEN; PRH; PRHX

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young