Hex (HHEX) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2H7]

CAT#: TA500027AM

Anti-Hex (Hex) mouse monoclonal antibody, clone OTI2H7 (formerly 2H7), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-Entry-HHex (RC204815, Right lane) cDNA for 48 hrs and lysed at 1:100 dilution. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HHex. Positive lysates LY419139 (100ug) and LC419139 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HHex monoclonal antibody at 1:50 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Hex antibody ( 2H7) at 1:100 dilution + Lysates from HEK-293T cells transfected with human Hex expression vector (Cat# RC204815)

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  Western blot analysis of extracts (10ug) from a mouse tissue by using anti-HHex monoclonal antibody (1:200).

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  Anti-HHex mouse monoclonal antibody (TA500027) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY HHex (RC204815)

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Specifications

Product Data

• Clone Name: OTI2H7
• Applications: IF, WB
• Recommended Dilution: WB: 1:50 - 1:100, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 100-270 of human Hex
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa (experimental 44 kDa)
• Gene Name: hematopoietically expressed homeobox
• Database Link:
  NP_002720
  Entrez Gene 15242 MouseEntrez Gene 79237 RatEntrez Gene 3087 Human
  UniProt ID Q03014
• Background: Hex is a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation.
• Synonyms: HEX; HMPH; HOX11L-PEN; PRH; PRHX

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young