Neurotrophin 3 (NTF3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3D4]

CAT#: TA500032AM

NT3 (Neurotrophin 3) mouse monoclonal antibody, clone OTI3D4 (formerly 3D4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF3 (RC214281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF3. Positive lysates LY420181 (100ug) and LC420181 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NTF3 monoclonal antibody.

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  NT3 antibody ( 3D4 ) at 1:10000 + Recombinant Human NT3 (Cat# RC214281)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NTF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500032)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NTF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500032)

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Specifications

Product Data

• Clone Name: OTI3D4
• Applications: IHC, WB
• Recommended Dilution: WB 1:5000~10000, IHC 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 139-257 of human NT3 (NP_002518) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.4 kDa
• Gene Name: neurotrophin 3
• Database Link:
  NP_001096124
  Entrez Gene 18205 MouseEntrez Gene 81737 RatEntrez Gene 4908 Human
  UniProt ID P20783
• Background: NT3 is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. It is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
• Synonyms: HDNF; NGF-2; NGF2; NT-3; NT3

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway