PDX1 Mouse Monoclonal Antibody [Clone ID: OTI2A12]

CAT#: TA500038S

Pdx1 mouse monoclonal antibody, clone OTI2A12 (formerly 2A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (RC222354, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1 (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500038)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500038)

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  Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY PDX1 (RC222354) using anti-PDX1 antibody (TA500038/green, left). 293T cells transfected with empty vector served as a negative control (right) (1:100).

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  Immunofluorescent staining of HT29 cells using anti-PDX1 mouse monoclonal antibody (TA500038).

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PDX1 monoclonal antibody (1:200).

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Specifications

Product Data

• Clone Name: OTI2A12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:20000, IHC 1:50, IF 1:50~100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDX1 (NP_000200) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.6 kDa
• Gene Name: pancreatic and duodenal homeobox 1
• Database Link:
  NP_000200
  Entrez Gene 18609 MouseEntrez Gene 29535 RatEntrez Gene 3651 Human
  UniProt ID P52945
• Background: Pdx1 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. It is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in PDX1 gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
• Synonyms: GSF; IDX-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; STF-1

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young, Type II diabetes mellitus