Carboxypeptidase A (CPA1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2A3]

CAT#: TA500053AM

Anti-CPA-1 (Carboxypeptidase A1) mouse monoclonal antibody, clone OTI2A3 (formerly 2A3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPA1 (RC202720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPA1. Positive lysates LY419696 (100ug) and LC419696 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-CPA1 (TA500053) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• Anti-CPA1 mouse monoclonal antibody (TA500053) immunofluorescent staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY CPA1 (RC202720).

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Specifications

Product Data

• Clone Name: OTI2A3
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC 1:50, IF (1:100)
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-419 of human CPA-1
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.5 kDa
• Gene Name: carboxypeptidase A1
• Database Link:
  NP_001859
  Entrez Gene 1357 Human
  UniProt ID P15085
• Background: Three different forms of human pancreatic procarboxypeptidase A have been isolated. The A1 and A2 forms are monomeric proteins with different biochemical properties. Carboxypeptidase A1 is a monomeric pancreatic exopeptidase. It is involved in zymogen inhibition
• Synonyms: CPA

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Secreted Protein