alpha 1 Antitrypsin (SERPINA1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1C2]

CAT#: TA500068BM

SERPINA1 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (RC202082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1. Positive lysates LY400112 (100ug) and LC400112 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500068)

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  HEK293T cells transfected with either RC202082 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINA1 antibody (TA500068), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1C2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 26-418 of human A1AT (NP_000286) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.6 kDa
• Gene Name: serpin family A member 1
• Database Link:
  NP_000286
  Entrez Gene 5265 Human
  UniProt ID P01009
• Background: A1AT is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in A1AT gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
• Synonyms: A1A; A1AT; AAT; alpha1AT; PI; PI1; PRO2275

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Complement and coagulation cascades