MAPK6 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A9]

CAT#: TA500076BM

Anti-ERK3 mouse monoclonal antibody, clone OTI1A9 (formerly 1A9), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6 (RC201597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6. Positive lysates LY400969 (100ug) and LC400969 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-ERK3 ([TA000076]) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Specifications

Product Data

• Clone Name: OTI1A9
• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC 1;50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.5 kDa
• Gene Name: mitogen-activated protein kinase 6
• Database Link:
  NP_002739
  Entrez Gene 50772 MouseEntrez Gene 58840 RatEntrez Gene 5597 Human
  UniProt ID Q16659
• Background: ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters
• Synonyms: ERK3; HsT17250; p97MAPK; PRKM6

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase