Neurogenin3 (NEUROG3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5A8]

CAT#: TA500090AM

Anti-Ngn3 (Neurogenin3) mouse monoclonal antibody, clone OTI5A8 (formerly 5A8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (RC224767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3. Positive lysates LY402827 (100ug) and LC402827 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (TA500090) at 1:150 dilution at 1:150 dilution.

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  Immunoprecipitation of NEUROG3 by using TrueMab monoclonal anti-NEUROG3 antibody (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Specifications

Product Data

• Clone Name: OTI5A8
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1:500, IHC: 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 1-214of human Ngn3
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.9 kDa
• Gene Name: neurogenin 3
• Database Link:
  NP_066279
  Entrez Gene 50674 Human
  UniProt ID Q9Y4Z2
• Background: Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm (Gradwohl et al., 2000 [PubMed 10677506]).[
• Synonyms: Atoh5; bHLHa7; Math4B; NGN-3; ngn3

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Maturity onset diabetes of the young