HNF6 (ONECUT1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4F12]

CAT#: TA500196AM

HNF6 mouse monoclonal antibody, clone OTI4F12 (formerly 4F12), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF6 (RC218219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF6. Positive lysates LY401429 (100ug) and LC401429 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HNF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500196)

• 

  Anti-HNF6 mouse monoclonal antibody (TA500196) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNF6 (RC218219).

Specifications

Product Data

• Clone Name: OTI4F12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 85-198 of human HNF6 (NP_004489) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.8 kDa
• Gene Name: one cut homeobox 1
• Database Link:
  NP_004489
  Entrez Gene 15379 MouseEntrez Gene 25231 RatEntrez Gene 3175 Human
  UniProt ID Q9UBC0
• Background: The protein encoded by HNF6 is Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a varietyof gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
• Synonyms: HNF-6; HNF6; HNF6A

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young