IL3 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5G7H9]

CAT#: TA500204AM

Anti-IL-3 mouse monoclonal antibody, clone OTI5G7H9 (formerly 5G7H9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-3 (RC210109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-3. Positive lysates LY424621 (100ug) and LC424621 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5G7H9
• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 20-152 of human IL-3
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.2 kDa
• Gene Name: interleukin 3
• Database Link:
  NP_000579
  Entrez Gene 3562 Human
  UniProt ID P08700
• Background: The protein encoded by IL-3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
• Synonyms: IL-3; MCGF; MULTI-CSF

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway