CDX2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1A3]

CAT#: TA500251AM

Anti-CDX2 mouse monoclonal antibody, clone OTI1A3 (formerly 1A3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDX2 (RC204883, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDX2. Positive lysates LY420044 (100ug) and LC420044 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDX2 monoclonal antibody.

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  Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-CDX2 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CDX2-Knockout 293T cells (KO, Cat# LC810043) were separated by SDS-PAGE and immunoblotted with anti-CDX2 monoclonal antibody TA500251. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

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  Immunofluorescent staining of 293T cells using anti-CDX2 mouse monoclonal antibody (TA500251, green, upper left; merged, upper right) or Isotype control ( merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).

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Specifications

Product Data

• Clone Name: OTI1A3
• Applications: IF, WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-313 of human CDX2
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.5 kDa
• Gene Name: caudal type homeobox 2
• Database Link:
  NP_001256
  Entrez Gene 12591 MouseEntrez Gene 66019 RatEntrez Gene 486028 DogEntrez Gene 1045 Human
  UniProt ID Q99626
• Background: The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730)and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription.
• Synonyms: AS; CDX-3; CDX2; CDX3

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors