GATA4 Mouse Monoclonal Antibody [Clone ID: OTI1H8]

CAT#: TA500253S

Anti-GATA4 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (RC210945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4. Positive lysates LY419558 (100ug) and LC419558 (20ug) can be purchased separately from OriGene.

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  Figure from citation: Western Blot of GATA4 protein level by using anti-GATA4 antibody in hESCs. View Citation

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  Anti-GATA4 mouse monoclonal antibody (TA500253) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA4 (RC210945).

Specifications

Product Data

• Clone Name: OTI1H8
• Applications: IF, WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 296-442of human GATA4
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1.1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.4 kDa
• Gene Name: GATA binding protein 4
• Database Link:
  NP_002043
  Entrez Gene 14463 MouseEntrez Gene 54254 RatEntrez Gene 2626 Human
  UniProt ID P43694
• Background: GATA4 encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects
• Synonyms: ASD2; TACHD; TOF; VSD1

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors